{"knowledge_schema": {"broad_category": "Biochemistry \u2192 Protein Structure and Function \u2192 Disulfide Bond Formation", "refinement": "This problem focuses on the identification of specific m/z values that correspond to the active form of a protein based on its disulfide bridge formation.", "specific_scope": "The task involves analyzing the mass-to-charge ratios (m/z) of peptide fragments resulting from trypsin digestion of the protein XER22, specifically looking for those that indicate the presence of the two required disulfide bridges for therapeutic activity.", "goal": "Determine the correct m/z values that confirm the presence of the active form of protein XER22 with the necessary disulfide bridges."}, "summary": "This problem involves understanding the relationship between protein structure, specifically disulfide bond formation, and the analysis of peptide fragments using mass spectrometry (LC/MS). The goal is to identify specific m/z values that indicate the presence of the active form of protein XER22, which requires two disulfide bridges. By analyzing the provided candidates, researchers can determine which m/z values confirm the successful production of the active therapeutic protein."}