{"knowledge_schema": {"broad_category": "Biological Sciences \u2192 Immunology \u2192 Cell Sorting and Antigen Interactions", "refinement": "This problem focuses on the efficiency of sorting double-positive tumor cell and B cell doublets to study antigen-specific interactions.", "specific_scope": "The context involves using a cell sorter to isolate specific cell doublets for downstream applications, such as RT-PCR, and analyzing the factors affecting the yield of paired heavy and light chains.", "goal": "Identify the reasons for low efficiency in obtaining paired heavy and light chains from sorted doublets."}, "summary": "The problem revolves around understanding the low efficiency of obtaining paired heavy and light chains from sorted double-positive tumor cell and B cell doublets. Several factors could contribute to this issue, including insufficient time for antigen interactions, the rarity of true antigen-specific interactions, inadequate incubation conditions for stable binding, inefficiencies in the cell sorting process, or potential RNA dominance from tumor cells. Analyzing these factors can help pinpoint the cause of the low yield."}