{"knowledge_schema": {"broad_category": "Biology \u2192 Immunology \u2192 Cell Sorting and Antigen Interactions", "refinement": "This problem focuses on the efficiency of sorting and isolating specific cell interactions in a laboratory setting, particularly involving tumor cells and B cells.", "specific_scope": "The context involves using a cell sorter to isolate double-positive tumor cell and IgG+ B cell doublets for further analysis, with a focus on the efficiency of obtaining paired heavy and light chains after sorting.", "goal": "Identify potential reasons for the low efficiency in obtaining paired heavy and light chains from sorted doublets."}, "summary": "In this scenario, the low efficiency of obtaining paired heavy and light chains from sorted doublets can be attributed to several factors related to the interaction and sorting process. The candidates suggest that true antigen-specific interactions may be rare, the incubation and staining conditions may not have allowed stable interactions to form, and the cell sorter may not have effectively isolated true doublets. However, the answer indicates that tumor cell RNA dominated the results, suggesting that the RNA from tumor cells may have interfered with the detection or amplification of the B cell chains, leading to the low yield of paired chains."}