{"knowledge_schema": {"broad_category": "Genetics \u2192 Genomic Sequencing \u2192 Allele Detection", "refinement": "This problem focuses on identifying low-frequency alleles in microbial populations, particularly those associated with drug resistance.", "specific_scope": "The task involves selecting the most efficient method for sequencing and analyzing microbial genomes to detect rare alleles with minimal steps.", "goal": "Identify very low frequency alleles responsible for drug resistance in a population of microbial cells using the least number of steps."}, "summary": "The problem involves identifying low-frequency alleles in microbial populations, specifically those linked to drug resistance. The goal is to find the most efficient method for sequencing and analyzing genomes to detect these rare alleles with minimal steps. The options provided include various combinations of library preparation, sequencing depth, and tools for alignment. The focus is on streamlining the process to achieve accurate detection of alleles with the least complexity."}