{"knowledge_schema": {"broad_category": "Biochemistry \u2192 Enzyme Regulation \u2192 Protein Kinase Activity", "refinement": "This problem focuses on the interaction and phosphorylation of a specific enzyme (GIKS3) by various kinases (CaPK1 to CaPK5) and the effects of these interactions on enzyme activity.", "specific_scope": "The context involves analyzing experimental data from SEC-MALS and phosphorylation assays to determine which kinases can activate GIKS3 by phosphorylating it at serine 25.", "goal": "Identify which kinases can phosphorylate GIKS3 on serine 25 and their ability to activate the enzyme based on experimental results."}, "summary": "This problem involves understanding the interactions between the enzyme GIKS3 and various calcium-dependent protein kinases (CaPK1 to CaPK5). The goal is to determine which kinases can phosphorylate GIKS3 at serine 25, leading to its activation. The experimental data shows that CaPK2 and CaPK3 can phosphorylate GIKS3, while CaPK1 and CaPK4 do interact but do not lead to activation. Based on the results, the correct answer is that only CaPK2 and CaPK3 can phosphorylate GIKS3 on serine 25."}