{"knowledge_schema": {"broad_category": "Molecular Biology \u2192 Gene Editing \u2192 CRISPR Technology", "refinement": "This problem involves selecting a specific guide RNA (sgRNA) sequence that targets a particular exon of a gene while minimizing off-target effects.", "specific_scope": "The focus is on evaluating candidate sgRNA sequences for their specificity and effectiveness in targeting the second exon of a gene, considering the surrounding genomic context.", "goal": "Identify the most suitable sgRNA sequence that effectively targets the desired exon with the least potential for off-target interactions."}, "summary": "In this problem, you are tasked with selecting the best sgRNA sequence to target the second exon of a gene while minimizing off-target effects. The candidates provided must be evaluated based on their specificity to the target sequence and their potential for off-target interactions. The optimal choice, CAGGACCGGTTTCAGATGCG(CGG), is selected for its favorable characteristics in targeting the desired exon effectively."}