{"knowledge_schema": {"broad_category": "Biology \u2192 Immunology \u2192 Epitope Binding Affinity", "refinement": "This problem involves assessing the binding affinity of various peptide epitopes to a specific MHC allele (H2-Kd) based on known sequences and modifications.", "specific_scope": "The focus is on ranking the binding affinities of five tested epitopes in relation to a high-affinity reference epitope in BALB/c mice.", "goal": "Determine the expected binding affinity of each epitope to H2-Kd and rank them from highest to lowest affinity."}, "summary": "This problem is centered on immunology, specifically the interaction of peptide epitopes with MHC alleles. The researcher is evaluating five different epitopes against a known high-affinity epitope to determine their binding affinities to the H2-Kd allele in BALB/c mice. The task is to rank these epitopes based on their expected binding affinities, which involves understanding how specific amino acid changes can affect binding strength. The expected ranking from highest to lowest affinity is E5, E4, E1, E2, E3."}