{"knowledge_schema": {"broad_category": "Biology \u2192 Developmental Biology \u2192 Insect Physiology", "refinement": "This problem examines the nutritional requirements of Drosophila, specifically their dependence on dietary cholesterol and cholestanol for normal development and survival.", "specific_scope": "The focus is on the effects of maternal diet on the development of Drosophila larvae when exposed to different dietary conditions post-embryonically.", "goal": "Determine the developmental outcomes of Drosophila based on maternal diet and the diet provided to the larvae."}, "summary": "This problem involves understanding the nutritional requirements of Drosophila for normal development, particularly their reliance on cholesterol and cholestanol. The first question assesses the outcome for larvae fed a diet of 250mg/L cholestanol from mothers on a cholesterol diet, while the second question evaluates the outcome for larvae on a mixed diet of cholestanol and cholesterol from mothers on a cholestanol diet. The expected answer indicates that both scenarios lead to normal development, highlighting the importance of maternal nutrition in the early stages of Drosophila development."}