{"knowledge_schema": {"broad_category": "Biochemistry \u2192 Nucleic Acids \u2192 Thermodynamics", "refinement": "This problem focuses on the limitations of experimental methods used to study nucleic acid thermodynamics, specifically through melting experiments in bulk.", "specific_scope": "The context is the nearest-neighbor base-pair model and how melting experiments can affect the accuracy of the derived thermodynamic parameters for nucleic acids.", "goal": "Identify the specific limitation of bulk melting experiments that could impact the results of nucleic acid thermodynamics."}, "summary": "The question addresses a limitation in the thermodynamic description of nucleic acids based on melting experiments in bulk. These experiments, while useful for determining thermodynamic parameters, face challenges that can affect the accuracy of the results. The goal is to identify the specific limitation among the provided options that impacts the reliability of the melting experiments."}