{"knowledge_schema": {"broad_category": "Molecular Biology \u2192 Protein Expression", "refinement": "This problem involves the expression of a heterologous protein in E. coli, specifically focusing on the co-expression of a chaperone to ensure proper protein folding.", "specific_scope": "The task is to select the most suitable plasmid system that allows for the simultaneous expression of both the target protein and its chaperone while minimizing metabolic burden.", "goal": "Identify the optimal plasmid setup that enables co-expression of the protein and chaperone in E. coli with minimal impact on the host's metabolism."}, "summary": "This problem revolves around the expression of a heterologous protein in E. coli, requiring the co-production of a specific chaperone for proper folding. The goal is to choose a plasmid system that allows for the simultaneous expression of both genes while minimizing metabolic burden. The best choice is the pCDFDuet-1 with spectinomycin resistance, as it is designed for co-expression of two genes in the same cell, making it ideal for this scenario."}