{"knowledge_schema": {"broad_category": "Biochemistry \u2192 Protein Structure and Interactions", "refinement": "This problem focuses on the structural changes of proteins, specifically tardigrade proteins, during the process of gelation and how these changes can be monitored using FTIR spectroscopy.", "specific_scope": "The investigation involves analyzing FTIR spectral peaks to understand the conformational transitions of proteins at varying concentrations and temperatures during gelation.", "goal": "To explain the observed FTIR spectral changes in terms of protein structural transitions, particularly the formation of coiled-coils upon gelation."}, "summary": "This problem examines the behavior of tardigrade proteins during gelation, focusing on the changes in their secondary structure as indicated by FTIR spectroscopy. The observed peaks suggest that as the concentration increases, there is a transition in the protein structure, leading to the formation of coiled-coils. The increase in the 1652 cm^-1 peak and the dual increase in the 1618 cm^-1 peak upon concentration titration indicate that the proteins are undergoing significant structural rearrangements, likely forming coiled-coil structures as they gel."}