{"knowledge_schema": {"broad_category": "Biology \u2192 Cell Culture \u2192 Fibroblast Isolation", "refinement": "This problem focuses on the techniques and conditions necessary for isolating and propagating corneal stromal fibroblasts from donor corneas.", "specific_scope": "The task involves understanding the correct procedures and media requirements for culturing corneal stromal fibroblasts after the removal of epithelial and endothelial layers.", "goal": "Identify the correct statement regarding the isolation and propagation of corneal stromal fibroblasts in vitro."}, "summary": "This question pertains to the isolation and culture of corneal stromal fibroblasts, which are crucial for studying corneal defects. The correct approach involves removing the epithelium and then using a specific serum-free medium, such as FibroLife, supplemented with appropriate factors to support the growth of the isolated stromal cells. Understanding the correct conditions for cell culture is essential for successful fibroblast propagation."}