{"knowledge_schema": {"broad_category": "Chemistry \u2192 Organic Chemistry \u2192 Photochemistry", "refinement": "This problem involves understanding the photochemical reactions and the role of specific molecules in fluorescence enhancement under light irradiation.", "specific_scope": "The focus is on identifying the molecule responsible for the observed difference in fluorescence when changing the probe in a specific experimental setup involving HEK 293T cell lysate and light irradiation.", "goal": "Determine which molecule among the candidates contributes to the fluorescent difference observed with the second probe."}, "summary": "This problem revolves around the photochemical behavior of specific organic compounds and their interaction with light to produce fluorescence. The experiment compares the fluorescent signals of two different probes under light irradiation, aiming to identify the molecule responsible for the observed differences in fluorescence. The goal is to analyze the candidates provided and determine which one contributes to the fluorescence change when the probe is altered."}