{"knowledge_schema": {"broad_category": "Biology \u2192 Molecular Biology \u2192 Fluorescence Microscopy", "refinement": "This problem involves understanding the principles of fluorescence and the specific excitation and emission wavelengths of fluorescent proteins used in biological imaging.", "specific_scope": "The focus is on identifying the correct excitation wavelength for detecting signals from fluorescent tags (HaloTag-eGFP and SNAPtag-DsRed) in a transgenic zebrafish model.", "goal": "Determine the appropriate excitation wavelength to visualize the fluorescent signals emitted by the tagged proteins in the zebrafish."}, "summary": "This question pertains to the use of fluorescence microscopy in biological research, specifically in the context of a transgenic zebrafish model. The task is to identify the correct excitation wavelength for detecting fluorescent signals from two different tags: HaloTag-eGFP and SNAPtag-DsRed. Understanding the excitation and emission properties of these fluorescent proteins is crucial for effective imaging. The correct answer, 630 nm, corresponds to the excitation wavelength needed to first detect the signals from the DsRed tag."}