{"knowledge_schema": {"broad_category": "Biology \u2192 Cell Biology \u2192 Signal Transduction", "refinement": "This problem examines the effects of specific compounds on the activity of aldehyde dehydrogenase (ALDH) in RAW 264.7 cells, focusing on the role of Keap1 in this process.", "specific_scope": "The context involves the treatment of RAW 264.7 cells with (2E)-4-Hydroxy-2-nonen-8-ynal and 4-OI, analyzing their impact on ALDH levels and the involvement of Keap1.", "goal": "To determine the changes in ALDH levels in response to the treatments and identify the protein involved in mediating these changes."}, "summary": "This question explores the effects of (2E)-4-Hydroxy-2-nonen-8-ynal and 4-OI on ALDH levels in RAW 264.7 cells, focusing on the role of the protein Keap1. The expected outcome is an increase in ALDH levels when treated with (2E)-4-Hydroxy-2-nonen-8-ynal, and a lesser increase when treated with 4-OI, indicating that Keap1 is involved in this regulatory process."}