{"knowledge_schema": {"broad_category": "Genetics \u2192 Microbial Genomics \u2192 Allele Detection", "refinement": "This problem focuses on identifying low-frequency alleles associated with drug resistance in microbial populations using sequencing techniques.", "specific_scope": "The task involves selecting the most efficient method for sequencing and analyzing microbial genomes to detect rare alleles.", "goal": "Identify very low frequency alleles in a population of microbial cells with minimal steps."}, "summary": "The problem involves identifying low-frequency alleles responsible for drug resistance in microbial cells. The goal is to find the most efficient method to achieve this with the least number of steps. The candidates suggest various approaches involving sequencing depth, library preparation, and the use of specific sequencing technologies like MinION. The final step in the process is to compare the sequenced reads to the alleles of interest, which is crucial for identifying the rare alleles effectively."}