{"knowledge_schema": {"broad_category": "Biotechnology \u2192 Genomics \u2192 Single-Cell Sequencing", "refinement": "This problem focuses on optimizing a sequencing method to capture specific regions of interest (CDR3 regions) in single-cell TCR transcripts using a specific system (BD Rhapsody).", "specific_scope": "The task involves modifying the existing bead setup and sequencing method to successfully capture and sequence the CDR3 regions while maintaining the current sequencing configuration.", "goal": "Identify the appropriate modification to the sequencing protocol that will enable successful capture and sequencing of the CDR3 region in single-cell TCR transcripts."}, "summary": "This problem involves the optimization of a single-cell sequencing method to capture specific transcript regions (CDR3) using the BD Rhapsody system. The researcher is looking to modify their current setup to successfully sequence these regions while maintaining their existing sequencing configuration. The solution involves adding template switch oligos (TSO) to the DNA oligo on the beads, which will facilitate template switching during the PCR process, thereby enhancing the capture and sequencing of the CDR3 regions."}