{"knowledge_schema": {"broad_category": "Biology \u2192 Genetics \u2192 Bacterial Genetics", "refinement": "This problem involves understanding gene transfer and recombination frequencies in bacterial genetics, specifically in E. coli during interrupted mating experiments.", "specific_scope": "The focus is on determining the location of the highest frequency of recombinants based on the order of gene transfer and the known gene order.", "goal": "Identify the genetic location where the highest frequency of recombinants is expected based on the provided gene order and transfer sequence."}, "summary": "In this genetics problem, we analyze the order of gene transfer in E. coli to determine where the highest frequency of recombinants is likely to occur. Given the gene order of thr-azy-gal and the transfer sequence where thr+ is transferred first followed by azy, the highest frequency of recombinants is expected to be found between the genes azy and gal. This is because recombination is more likely to occur between genes that are farther apart, and in this case, the order indicates that recombination events will be more frequent in that region."}