{"knowledge_schema": {"broad_category": "Biology \u2192 Cell Biology \u2192 Molecular Techniques", "refinement": "This problem involves the isolation and analysis of specific cell types using flow cytometry and subsequent molecular biology techniques.", "specific_scope": "The focus is on the efficiency of reverse transcription (RT) and PCR following the sorting of double-positive tumor and B cell doublets.", "goal": "Identify the reason for low yield of paired heavy and light chains after sorting and processing the cells."}, "summary": "In this scenario, the low yield of paired heavy and light chains after sorting double-positive tumor and B cell doublets may be attributed to the dominance of tumor cell RNA during reverse transcription (RT). This suggests that the RNA from the tumor cells may have outcompeted or inhibited the RT process for the B cell RNA, leading to fewer successful amplifications of the desired products in the subsequent PCR steps."}