{"knowledge_schema": {"broad_category": "Biochemistry \u2192 Protein Structure \u2192 Protein Folding and Aggregation", "refinement": "This problem examines the structural transitions of proteins, particularly disordered proteins, in response to hydration and temperature changes.", "specific_scope": "The focus is on the behavior of gel-forming tardigrade intrinsically disordered proteins as observed through FTIR spectroscopy, analyzing specific amide I bands and their changes under different conditions.", "goal": "Identify the structural transition that best explains the observed FTIR spectral changes in the context of protein aggregation and gelation."}, "summary": "This problem involves understanding the structural transitions of tardigrade intrinsically disordered proteins as they undergo hydration and heating, analyzed through FTIR spectroscopy. The observed changes in amide I bands suggest specific interactions and structural formations. The goal is to determine which structural transition\u2014such as the formation of coiled-coils\u2014best explains the spectral data, particularly the growth of the 1645 cm\u22121 band and the behavior of the other bands during concentration titration and heating."}