{"knowledge_schema": {"broad_category": "Biochemistry \u2192 Enzyme Kinetics \u2192 Assay Troubleshooting", "refinement": "This problem involves understanding the factors that can affect enzyme activity and the linearity of product formation in an enzyme kinetics assay.", "specific_scope": "The focus is on troubleshooting an enzyme kinetics assay where the product formation does not show a linear phase, despite certain controlled conditions being met.", "goal": "Identify the appropriate action to take in order to achieve a linear phase in the Product vs Time plot of the enzyme kinetics assay."}, "summary": "In troubleshooting an enzyme kinetics assay where the product formation does not show a linear phase, it is important to consider factors affecting enzyme activity. Given that the enzyme functions as an obligate dimer and the assay conditions are controlled, the most logical step is to increase the enzyme concentration. This can help ensure that the reaction proceeds at a rate that allows for a linear increase in product formation, as a higher enzyme concentration can enhance the reaction rate, potentially leading to a more linear response in the Product vs Time plot."}