{"knowledge_schema": {"broad_category": "Biochemistry \u2192 Enzyme Kinetics \u2192 Enzyme Regulation", "refinement": "This problem examines the effects of different molecules on the activity of the enzyme Ytr2, specifically focusing on how these molecules influence the enzyme's catalytic efficiency (kcat).", "specific_scope": "The context involves analyzing the impact of various metal ions and compounds on the enzyme's activity to determine their roles as activators or inhibitors.", "goal": "Identify the functions of molecules Act1 and Inh1 based on their effects on the enzyme's activity as indicated by the kcat values."}, "summary": "This problem involves understanding enzyme kinetics and regulation, specifically how different molecules affect the activity of the enzyme Ytr2. The results show that Act1 significantly increases the enzyme's activity, suggesting it acts as an allosteric activator, while Inh1 drastically reduces the activity, indicating it functions as an irreversible inhibitor. The goal is to analyze the kcat values to determine the roles of these molecules in enzyme regulation."}