{"knowledge_schema": {"broad_category": "Molecular Biology \u2192 Genetics \u2192 Codon-Anticodon Interactions", "refinement": "This problem examines the effects of mutations in tRNA anticodons on protein synthesis and the implications for the genetic code.", "specific_scope": "The focus is on a specific mutation in a tRNA anticodon that alters the amino acid incorporation during translation, leading to a rare amino acid substitution in proteins.", "goal": "To understand how the mutation in the tRNA anticodon affects the genetic code and the resulting protein product."}, "summary": "The problem involves a mutation in a tRNA anticodon that leads to a rare amino acid substitution in proteins. This mutation suggests that the altered tRNA can still pair with mRNA codons, potentially allowing for the incorporation of an amino acid that is normally inserted by a different anticodon. The correct implication of this substitution is that it allows insertion of an amino acid usually inserted by another, more common anticodon, which corresponds to option 3. This highlights the flexibility of the genetic code and the role of wobble pairing in tRNA-mRNA interactions."}