{"knowledge_schema": {"broad_category": "Biotechnology \u2192 Molecular Biology \u2192 Protein Expression", "refinement": "This problem focuses on the co-expression of proteins in E. coli, specifically the need for chaperone proteins to assist in the correct folding of heterologous proteins.", "specific_scope": "The task is to identify the best combination of plasmids that allows for the co-expression of a protein of interest and its required chaperone in E. coli, considering antibiotic resistance markers for selection.", "goal": "Select the optimal plasmid combination that enables effective co-expression of the chaperone and the target protein in E. coli."}, "summary": "This problem involves molecular biology techniques related to protein expression in E. coli, particularly the need for chaperone proteins to ensure proper folding of heterologous proteins. The task is to determine the best combination of plasmids that allows for the co-expression of a target protein and its chaperone, taking into account the antibiotic resistance markers for selection. The goal is to identify the optimal plasmid pair that facilitates this co-expression effectively."}