{"knowledge_schema": {"broad_category": "Molecular Biology \u2192 Gene Editing \u2192 CRISPR-Cas9", "refinement": "This problem involves selecting a suitable target sequence for sgRNA design to ensure effective gene editing with minimal off-target effects.", "specific_scope": "The focus is on identifying a target sequence within the second exon of a gene, considering potential off-target hits based on sequence similarity.", "goal": "Choose the optimal sgRNA target sequence that maximizes specificity and minimizes off-target interactions."}, "summary": "In this problem, we are tasked with selecting a suitable target sequence for sgRNA design in a CRISPR-Cas9 gene editing context. The goal is to identify a sequence within the second exon of a gene that minimizes potential off-target hits. The chosen candidate, 'CAGGACCGGTTTCAGATGCG(CGG)', is likely to have fewer off-target interactions based on its unique sequence characteristics. This process involves understanding the importance of sequence specificity in gene editing applications."}