{"knowledge_schema": {"broad_category": "Biology \u2192 Genetics \u2192 Bacterial Genetics", "refinement": "This problem involves understanding gene transfer and recombination frequency in bacterial genetics, specifically in E. coli.", "specific_scope": "The focus is on determining the location of the highest frequency of recombinants based on the order of gene transfer and the known gene order.", "goal": "Identify the location where recombination is most likely to occur based on the provided gene order and transfer sequence."}, "summary": "In this problem, we are analyzing the genetic mapping of E. coli based on interrupted mating experiments. The order of gene transfer is crucial for determining where recombination occurs most frequently. Given the gene order of thr-azi-gal and knowing that thr+ is transferred first followed by azy, we need to identify the location with the highest frequency of recombinants. The highest frequency of recombinants is expected to be between thr+ and azy, as this is where the genes are closest together and recombination is more likely to occur."}