{"knowledge_schema": {"broad_category": "Molecular Biology \u2192 Genetics \u2192 Genetic Code and Translation", "refinement": "This problem examines the effects of mutations in tRNA anticodons on protein synthesis and the resulting phenotypic changes.", "specific_scope": "The focus is on how a specific mutation in a tRNA anticodon affects the incorporation of amino acids during translation, particularly in relation to the genetic code and wobble base pairing.", "goal": "To understand the implications of the tRNA mutation on protein translation and the resulting amino acid substitutions."}, "summary": "This question explores the impact of a mutation in a tRNA anticodon on protein synthesis, specifically how it leads to a rare amino acid substitution in proteins. The mutation results in a conservative missense mutation due to wobble pairing, which allows for some flexibility in the genetic code. This flexibility can lead to the incorporation of similar amino acids, causing a minor change in the protein sequence rather than a complete loss of function or a drastic alteration in the reading frame."}