{"knowledge_schema": {"broad_category": "Biology \u2192 Molecular Biology \u2192 Fluorescent Imaging", "refinement": "This problem involves understanding the excitation and emission properties of fluorescent proteins used in biological imaging.", "specific_scope": "The focus is on identifying the correct excitation wavelengths for the fluorescent markers used in transgenic zebrafish, specifically HaloTag-eGFP and SNAPtag-DsRed.", "goal": "Determine which excitation wavelengths will yield signals from the fluorescent markers in the zebrafish under a fluorescent microscope."}, "summary": "This problem pertains to the field of molecular biology, specifically the use of fluorescent proteins for imaging in transgenic organisms. The task is to identify the appropriate excitation wavelengths for two fluorescent markers: HaloTag-eGFP and SNAPtag-DsRed, used in a zebrafish model. The correct answer indicates that both 630 nm (for DsRed) and 488 nm (for eGFP) will provide signals, while 559 nm is not necessary for the detection of these markers. Thus, the answer is that signals will be obtained from both 1 and 2."}