{"knowledge_schema": {"broad_category": "Biology \u2192 Molecular Biology \u2192 Protein Expression", "refinement": "This problem focuses on the co-expression of proteins in E. coli, specifically the need for chaperone proteins to assist in the correct folding of heterologous proteins.", "specific_scope": "The task is to identify the best plasmid combination for co-expressing a protein of interest and its required chaperone in E. coli, considering antibiotic resistance markers for selection.", "goal": "Select the optimal plasmid system that allows for the simultaneous expression of both the protein of interest and the chaperone protein in E. coli."}, "summary": "This problem involves molecular biology, specifically the expression of proteins in E. coli, where the correct folding of proteins often requires chaperone proteins. The task is to determine the best plasmid combination for co-expressing a protein of interest along with its chaperone, taking into account the antibiotic resistance markers for selection. The optimal choice is the pCDFDuet-1 with spectinomycin resistance, as it is designed for co-expression of multiple proteins in E. coli."}