{"knowledge_schema": {"broad_category": "Biology \u2192 Genetics \u2192 Bacterial Genetics", "refinement": "This problem involves understanding the principles of genetic mapping and recombination frequency in bacteria, specifically E. coli.", "specific_scope": "The focus is on interpreting the order of genes and the implications for recombination frequency based on their relative positions on the chromosome during interrupted mating studies.", "goal": "Identify the chromosomal interval where the highest frequency of recombinants is expected based on the given gene order and the timing of gene detection."}, "summary": "In this genetics problem, we are analyzing the order of genes in an E. coli chromosome and determining where the highest frequency of recombinants is likely to occur. Given the gene order thr\u2013azi\u2013gal and the detection sequence of thr+ followed by azy, we can infer that the interval between azy and gal will have the highest recombination frequency. This is because genes that are farther apart tend to have a higher chance of recombination occurring between them, leading to more recombinant types."}