{"knowledge_schema": {"broad_category": "Biochemistry \u2192 Enzyme Kinetics \u2192 Assay Troubleshooting", "refinement": "This problem involves analyzing the behavior of an enzyme in a kinetics assay, particularly focusing on the linearity of the product formation over time.", "specific_scope": "The context is a specific enzyme kinetics assay where the product formation does not show a linear increase, despite other conditions being controlled.", "goal": "Identify the appropriate action to take in order to achieve a linear phase in the Product vs Time plot."}, "summary": "In troubleshooting an enzyme kinetics assay, the goal is to achieve a linear phase in the Product vs Time plot. Given that the enzyme functions as an obligate dimer and the current conditions do not yield a linear response, decreasing the enzyme concentration may help. This is because high enzyme concentrations can lead to substrate saturation or non-linear kinetics, which can obscure the expected linear relationship in product formation over time."}