{"knowledge_schema": {"broad_category": "Molecular Biology \u2192 Gene Editing \u2192 CRISPR-Cas9", "refinement": "This problem involves selecting a suitable target sequence for sgRNA design to ensure specificity and minimize off-target effects.", "specific_scope": "The focus is on identifying a target sequence within the second exon of a gene, considering potential off-target hits based on sequence similarity.", "goal": "Choose the best candidate sequence for sgRNA that maximizes specificity and minimizes off-target interactions."}, "summary": "In this problem, you need to select a suitable target sequence for sgRNA design within the second exon of a gene, using the CRISPR-Cas9 system. The goal is to find a sequence that minimizes potential off-target hits, which can be achieved by analyzing the provided candidate sequences for uniqueness and specificity. The best candidate will be one that has the least similarity to other regions in the genome, thereby reducing the likelihood of unintended edits."}