Fix sample on MALDI target holder with superglue, perform 3D optical microscopy. #####%%%%% output "DHB" are used in line4
{"action": "correct", "output": "DHB", "reagent": ["superglue"]}
Dissolve DHB (30 mg/ml) .
{"action": "dissolve", "output": "", "reagent": ["DHB"]}
Apply 100 µl matrix solution using a pneumatic sprayer system at 10 µl/min bar. #####%%%%% output "known DHB cluster signals" are used in line10
{"action": "apply", "output": "known DHB cluster signals", "device": ["a pneumatic sprayer system"]}
Check matrix layer quality with an optical microscope.
{"action": "check", "output": "matrix layer quality", "device": ["optical microscope"]}
Calibrate mass spectrometer with known DHB cluster signals.
{"action": "calibrate", "output": "", "reagent": ["known DHB cluster signals"]}
Set mass spectrometer to m/z 100-1000, fixed injection time of 500 ms, .
{"action": "set", "output": "", "time": ["500 ms"], "device": ["mass spectrometer"]}
apply 4 kV to the MALDI target
{"action": "apply", "output": "", "device": ["MALDI target"], "voltage": ["4 kV"]}
Adjust laser pulse energy.
{"action": "adjust", "output": "", "device": ["laser pulse energy"], "time": [""]}
Start mass spectrometer data acquisition .
{"action": "start", "output": "", "device": ["mass spectrometer data acquisition"]}
initialize 3D scan
{"action": "initialize", "output": "", "device": ["3D scan"]}
Load data in MIRION .
{"action": "load", "output": "", "device": ["MIRION"]}
Set image bin width to m/z ±0.01.
{"action": "set", "output": "", "length": ["m"]}
Use 3D imaging data to display sample topography in heat map color scale using MATLAB.
{"action": "use", "output": "", "device": ["3D imaging data"]}
Combine three ion images into an RGB overlay image . #####%%%%% output "three ion images" are not used
{"action": "combine", "output": "three ion images", "device": [""]}
integrate with topographic information into 3D RGB MS surface images
{"action": "integrate", "output": "3D RGB MS surface images"}
Normalize MS images to the base pixel per image. #####%%%%% output "image" are not used
{"action": "normalize", "output": "image", "mass": [""]}