Procedural Description Subcutaneously inject 1 μl / g body weight of 0.5 mg / ml atropine sulfate monohydrate (Cat# 11330, Fluka, USA) into each mouse to prevent the salivary secretions from disrupting the movement of the infusions. #####%%%%% output "the infusions" are used in line12
{"action": "inject", "output": "the infusions", "reagent": ["atropine sulfate monohydrate"]}
Place the mouse on a custom-made plastic platform in the ventral position: the maxillary incisors are locked on a metal wire,
{"action": "place", "output": "", "device": ["custom-made plastic platform"]}
the mandibular incisors are hooked on an elastic string in order to hold the mouth open.
{"action": "connect", "output": "", "time": [""]}
Use an Insulin syringe with a 29-gauge needle Cat# 600145, placed inside a 0.58 mm diameter polyethylene tube (Cat# 427410, Becton Dickinson, ; Fig 2).
{"action": "use", "output": "", "device": ["Insulin syringe"]}
The polyethylene tube is inserted 5mm over the tip of a 29-gauge needle.
{"action": "insert", "output": "", "length": ["5mm"], "device": ["a 29-gauge needle"]}
The needle is used as a rigid guide to move the polyethylene tube into the Wharton's duct.
{"action": "use", "output": "", "reagent": ["the infusions"]}
The polyethylene tube is inserted 3-5 mm inside the duct.
{"action": "insert", "output": "", "container": ["the duct"], "device": [""]}
Note that only the polyethylene tube enters the salivary gland duct while the needle remains outside the duct.
{"action": "note", "output": "", "reagent": [""]}
The infusion is delivered steadily with positive pressure on the syringe piston; the finger remains on the piston even after all of the infusion has been delivered to the gland, in order to prevent a backflow of the infusion intra-orally. #####%%%%% output "the infusion" are used in line22
{"action": "continue", "output": "the infusion", "device": ["the syringe piston"]}
The trypan blue solution is used in the video as a dye to demonstrate the technique. #####%%%%% output "the technique" are not used
{"action": "use", "output": "the technique", "reagent": ["a dye"]}
fter the injection of the trypan blue solution, the mouse was euthanized prior to dissection. #####%%%%% output "dissection" are used in line28
{"action": "leave", "output": "dissection", "reagent": ["trypan blue", "the infusion"]}
The salivary gland is surgically exposed in order to determine how much of the infusions have actually remained in the gland.
{"action": "expose", "output": "", "reagent": ["the infusions"]}
Representative Results: After the infusions were injected, the mouse was dissected to surgically expose the post-cannulated salivary gland. #####%%%%% output "the post-cannulated salivary gland" are used in line36
{"action": "examine", "output": "the post-cannulated salivary gland", "time": [""]}
As shown on Image-2, the dark coloration of the Wharton's duct provides evidence that the trypan blue solution was infused successfully. #####%%%%% output "evidence that the trypan blue solution was infused successfully" are used in line32
{"action": "provide", "output": "evidence that the trypan blue solution was infused successfully", "reagent": ["dissection"]}
Polyethylene tube is encased around the tip of a 29-gauge needle.
{"action": "attach", "output": "", "device": ["a 29-gauge needle"]}
The syringe needle is used as a rigid guide to move the polyethylene tube into the Wharton's duct.
{"action": "use", "output": "", "reagent": ["evidence that the trypan blue solution was infused successfully"]}
The polyethylene tube is inserted (cannulated) 3-5 mm inside the duct.

Note that only the polyethylene tube enters the salivary gland duct while the needle remains outside the duct.
{"action": "note", "output": "", "reagent": ["the post-cannulated salivary gland"]}
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