{"action": "add", "output": "", "reagent": ["exo-resistant"], "volume": ["1 \u00b5l"]}
{"action": "keep", "output": "\u00b5l stop buffer (400 mM HCl, 600 mM Tris-HCl pH 7.5)", "reagent": [""], "temperature": ["ice"], "time": ["10 min"]}
{"action": "add", "output": "", "reagent": ["\u00b5l stop buffer (400 mM HCl, 600 mM Tris-HCl pH 7.5)"], "volume": ["3 \u00b5l"]}
{"action": "keep", "output": "2 \u00b5l Phi29 polymerase", "reagent": [""], "temperature": ["ice"], "time": ["2 min"]}
{"action": "combine", "output": "", "reagent": ["2 \u00b5l Phi29 polymerase"], "volume": ["32 \u00b5l master mix 30 \u00b5l MDA reaction buffer,"]}
{"action": "remove", "output": "clear liquid", "reagent": [""]}
{"action": "rinse", "output": "", "reagent": ["beads"]}
{"action": "minimize", "length": [""], "output": "nuclease-free water"}
{"action": "elute", "output": "", "reagent": ["nuclease-free water"], "volume": ["32 \u00b5l"]}
{"action": "use", "device": ["magnetic separator"], "output": ""}
{"action": "transfer", "container": ["tube"], "output": "", "reagent": ["clear liquid"], "volume": ["30 \u00b5l"]}
{"action": "quantify", "mass": ["10-12 \u00b5g"], "output": "TBE,", "reagent": ["", ""]}
{"action": "check", "device": ["agarose gel electrophoresis"], "output": "product size"}
{"action": "prepare", "container": ["96-well plate"], "output": "", "reagent": ["PCR mixture", "product size"]}
{"action": "incubate", "device": ["PCR machine"], "output": "", "temperature": ["95\u00b0C"], "time": ["20s"]}
{"action": "calculate", "output": "relative uniformity values (RUVs)"}