{"action": "grow", "output": "one tumor", "reagent": ["Lewis Lung Cancer (LLC)"]}
{"action": "place", "container": ["a 100 mm Petri dish"], "output": "", "reagent": ["one tumor"]}
{"action": "add", "output": "tumors", "temperature": ["RT"], "volume": ["5-10 ml HBSS"]}
{"action": "mince", "container": ["a 5 ml pipette"], "output": "tumor tissue suspension", "reagent": ["tumors"]}
{"action": "transfer", "container": ["50 ml non-vented tissue culture flasks"], "output": "", "reagent": ["tumor tissue suspension"]}
{"action": "rinse", "output": "", "volume": ["up to 40 ml"]}
{"action": "transfer", "container": ["the suspension"], "output": ""}
{"action": "add", "container": ["the flask"], "output": "", "volume": ["5 ml"]}
{"action": "shake", "device": ["shaker"], "output": "", "speed": ["80 rpm"], "temperature": ["RT"], "time": ["1-3 h"]}
{"action": "centrifuge", "force": ["50 x g"], "output": "cells", "temperature": ["RT"], "time": ["10 min"]}
{"action": "collect", "container": [""], "output": "the supernatant"}
{"action": "discard", "container": ["the tube"], "output": ""}
{"action": "centrifuge", "force": ["200 x g"], "output": "the supernatant", "time": ["5 min"]}
{"action": "suspend", "output": "", "reagent": ["cell pellet"], "time": ["1 min"], "volume": ["2 ml"]}
{"action": "add", "output": "cells", "reagent": ["more ACK lysing buffer"]}
{"action": "re-suspend", "output": "", "reagent": ["cells"], "volume": ["15 ml"]}
{"action": "add", "container": ["a 50 ml tube"], "output": "wash buffer", "reagent": ["cells"]}
{"action": "collect", "output": ""}
{"action": "wash", "output": "", "reagent": ["wash buffer"]}
{"action": "centrifuge", "force": ["425 x g"], "output": "tumor", "temperature": ["RT"], "time": ["10 min"]}
{"action": "note", "device": ["centrifugation"], "output": "", "reagent": ["tumor"]}
{"action": "re-suspend", "device": ["flow cytometry"], "output": "", "reagent": ["cells"]}
{"action": "see", "output": ""}
{"action": "see", "output": ""}