{"action": "add", "container": ["plasma"], "output": "", "reagent": ["Chloroform:Methanol:Isopropanol (2:1:1)"]}
{"action": "discard", "container": [""], "output": "the supernatant"}
{"action": "add", "device": [""], "output": "", "reagent": ["TCA/Acetone (50%)"], "volume": ["800 \u00b5l"]}
{"action": "incubate", "output": "", "temperature": ["-20\u00b0"]}
{"action": "discard", "container": [""], "output": "the supernatant"}
{"action": "add", "output": "", "reagent": ["40% Ammonium sulfate"]}
{"action": "incubate", "output": "", "temperature": ["ice"], "time": ["30 minutes"]}
{"action": "remove", "container": [""], "output": "the supernatant"}
{"action": "clean", "device": ["Bio-Rad clean-up kit"], "output": "the pellet"}
{"action": "dissolve", "output": "", "reagent": ["Rehydration buffer"], "volume": ["150-250 \u00b5l"]}
{"action": "resolve", "concentration": ["10%"], "container": ["SDS-PAGE"], "length": [""], "output": "", "reagent": ["the pellet"]}