{"action": "transfer", "output": "buffer", "reagent": ["serum"], "container": ["<<<a 1.5 ml microfuge tube>>>"]}
{"action": "add", "output": "the sample/XBP mixture", "reagent": ["<<<buffer>>>"]}
{"action": "add", "output": "buffer XWP", "reagent": ["the sample/XBP mixture"]}
{"action": "add", "output": "", "reagent": ["<<<buffer XWP>>>"], "volume": ["<<<10 ml>>>"]}
{"action": "transfer", "output": "Qiazol", "container": ["the spin column", "a fresh collection tube"], "volume": [""]}
{"action": "add", "output": "", "reagent": ["<<<Qiazol>>>"], "volume": ["700 \u03bcl"]}
{"action": "transfer", "output": "the flow-though", "container": ["a PLG tube.<sup>1</sup> 13"]}
{"action": "incubate", "output": "", "temperature": ["<<<room temperature>>>"], "time": ["<<<5 minutes>>>"]}
{"action": "add", "output": "", "volume": ["90 \u00b5l chloroform"], "mass": [""]}
{"action": "shake", "output": "", "time": ["<<<15 seconds>>>"]}
{"action": "incubate", "output": "", "temperature": ["<<<room temperature>>>"], "time": ["3 min"]}
{"action": "transfer", "output": "", "container": ["<<<a new microcentrifuge tube>>>"]}
{"action": "measure", "output": "", "container": ["the aqueous phase"]}
{"action": "add", "output": "", "reagent": ["<<<100% ethanol>>>"]}
{"action": "assemble", "output": "", "container": ["a MinElute spin column"], "reagent": [""]}
{"action": "load", "output": "", "volume": ["up to 700"], "device": [""], "reagent": ["the flow-though"]}
{"action": "move", "output": "buffer RWT", "time": ["23-25"]}
{"action": "add", "output": "", "reagent": ["<<<buffer RWT>>>"], "volume": ["700 \u00b5l"]}
{"action": "remove", "output": "the flow-through", "volume": [""]}
{"action": "remove", "output": "the flow-through", "volume": [""]}
{"action": "remove", "output": "the flow-through.<sup>4</sup> 36", "container": ["the collection tube"], "volume": [""], "reagent": ["the flow-through"]}
{"action": "transfer", "output": "", "device": ["RNeasy MinElute"], "container": ["a new 2 ml collection tube (supplied)"], "volume": [""]}
{"action": "open", "output": "", "container": ["<<<the spin column>>>"]}
{"action": "centrifuge", "output": "", "speed": ["full speed (14,000 xg)"], "container": ["membrane.<sup>5</sup>"], "time": ["<<<5 min>>>"]}
{"action": "remove", "output": "the flow-through.<sup>4</sup> 39", "container": ["the collection tube"], "volume": [""], "reagent": ["the flow-through.<sup>4</sup> 36"]}
{"action": "transfer", "output": "RNase-free water", "device": ["RNeasy MinElute"], "container": ["a new 1.5 ml collection tube (supplied)"], "volume": [""]}
{"action": "add", "output": "", "reagent": ["<<<RNase-free water>>>"], "volume": ["14 \u00b5l"]}