{"action": "extract", "output": "total RNA", "reagent": ["<<<TRIZOL reagent>>>"], "volume": ["<<<at least 2 x 10^6 cells>>>"], "container": [""]}
{"action": "reverse-transcribe", "output": "cDNA", "reagent": ["<<<total RNA>>>"]}
{"action": "prepare", "output": "cDNA", "reagent": ["2X Sybr Green PCR Master Mix", "primer mix", "cDNA"]}
{"action": "perform", "output": "", "device": ["<<<Real-Time PCR>>>"], "reagent": ["<<<cDNA>>>"], "volume": ["<<<10 ng>>>"]}
{"action": "conduct", "output": ""}
{"action": "analyze", "output": "", "device": ["built-in SDS Analysis Software"]}
{"action": "adjust", "output": ""}