{"action": "sow", "output": "", "container": ["Surface-sterilized"], "temperature": [""]}
{"action": "keep", "output": "", "temperature": ["<<<4 \u00b0C>>>"]}
{"action": "transfer", "output": "", "container": ["the growth chamber"], "temperature": ["<<<22 \u00b0C>>>"]}
{"action": "use", "output": "intracellular pH", "reagent": [""]}
{"action": "place", "output": "", "reagent": ["<<<liquid growth medium>>>"]}
{"action": "monitor", "output": "F488", "device": ["<<<confocal microscope>>>"]}
{"action": "operate", "output": "the calibration curve", "reagent": ["pH buffer solutions", "the buffer solution", "F488"], "device": [""]}
{"action": "recover", "output": "", "time": ["about 10 min"], "container": ["the external pH buffer solutions"], "temperature": [""], "volume": [""]}
{"action": "make", "output": "F405-to-[F488] ratio", "concentration": [""], "device": ["<<<fluorescence>>>"]}
{"action": "make", "output": "", "volume": [""]}
{"action": "confirm", "output": "in situ titration", "device": ["the Microsoft Excel"], "reagent": ["the calibration curve"]}
{"action": "calibrate", "output": ""}