{"action": "make", "output": "", "reagent": ["double-stranded", "salmon sperm DNA 1"], "length": [""]}
{"action": "add", "output": "", "volume": ["\u03bcL"], "container": ["<<<96-well>>>"]}
{"action": "wrap", "output": "", "length": [""], "reagent": [""]}
{"action": "incubate", "output": "the coating antibody solution", "temperature": ["4 \u00b0C"]}
{"action": "remove", "output": "1x PBS-Tween", "reagent": ["the coating antibody solution"]}
{"action": "wash", "output": "", "reagent": ["1x PBS-Tween"]}
{"action": "rinse", "output": "", "container": ["<<<the plate>>>"]}
{"action": "add", "output": "", "container": ["well"]}
{"action": "incubate", "output": "the blocking solution", "container": ["the plate"], "temperature": ["room temperature (RT)"], "time": [""]}
{"action": "remove", "output": "1x PBS-Tween", "reagent": ["the blocking solution"]}
{"action": "wash", "output": "", "reagent": ["1x PBS-Tween"]}
{"action": "rinse", "output": "", "container": ["the plate"]}
{"action": "keep", "output": "the spleen", "temperature": ["4 \u00b0C"]}
{"action": "export", "output": "spleen pieces", "reagent": ["the spleen", "the spleen"], "time": [""]}
{"action": "create", "output": "a single-cell suspension", "reagent": ["spleen pieces"]}
{"action": "transfer", "output": "", "container": ["the cells"]}
{"action": "turn", "output": "", "temperature": ["4 \u00b0"]}
{"action": "suspend", "output": "", "container": ["the cells"], "concentration": ["0.17 M ammonium chloride"]}
{"action": "keep", "output": "", "time": ["5 min"]}
{"action": "remove", "output": "the supernatant", "reagent": ["a single-cell suspension"]}
{"action": "suspend", "output": "", "reagent": ["DMEM", "the supernatant"]}
{"action": "estimate", "output": "the cells", "reagent": [""]}
{"action": "replace", "output": "", "concentration": ["10%"]}
{"action": "add", "output": "", "volume": ["50"], "container": ["DNA-coated"]}
{"action": "incubate", "output": "", "temperature": ["30 \u00b0C"], "time": ["2 h"]}
{"action": "add", "output": "", "volume": ["50"], "container": ["the cells"]}
{"action": "centrifuge", "output": "CO2", "container": ["the cells"], "force": ["300 RCF"], "temperature": ["<<<4 \u00b0C>>>"], "reagent": [""], "time": [""]}
{"action": "incubate", "output": "", "reagent": ["CO2"], "temperature": [""], "time": [""]}
{"action": "remove", "output": "", "container": ["the cells"], "volume": [""]}
{"action": "wash", "output": "", "time": [""], "reagent": ["the cells"]}
{"action": "rinse", "output": "streptavidin alkaline phosphatase (1:1,000 [in 1]% BSA/PBS)", "container": ["the plates"]}
{"action": "add", "output": "", "reagent": ["streptavidin alkaline phosphatase 1:1,000 [in 1]% BSA/PBS"], "container": ["the plate"]}
{"action": "incubate", "output": "10x PBS-Tween 20", "container": ["the plate"], "temperature": ["RT"], "time": ["1 h"]}
{"action": "wash", "output": "", "reagent": ["10x PBS-Tween 20"]}
{"action": "rinse", "output": "AMP buffer", "container": ["the plate"]}
{"action": "add", "output": "the plate", "reagent": ["<<<AMP buffer>>>"]}