A robust peak detection method for RNA structure inference by high-throughput contact mapping

Jinkyu Kim, Seunghak Yu, Byonghyo Shim, Hanjoo Kim, Hyeyoung Min, Eui-Young Chung, Rhiju Das, Sungroh Yoon

Abstract: For high-throughput prediction of the helical arrangements of large RNA molecules, an innovative method termed multiplexed hydroxyl radical (·OH) cleavage analysis (MOHCA) has been proposed. A key step in this promising technique is to detect peaks accurately from noisy radioactivity profiles. Since manual peak finding is laborious and prone to error, an automated peak detection method to improve the accuracy and throughput of MOHCA is required. Existing methods were not applicable to MOHCA due to their high false positive rates.