Artificial immunofluorescence in a flash: Rapid synthetic imaging from brightfield through residual diffusion

Xiaodan Xing, Chunling Tang, Siofra Murdoch, Giorgos Papanastasiou, Yunzhe Guo, Xianglu Xiao, Jan Oscar Cross-Zamirski, Carola-Bibiane Schönlieb, Kristina Xiao Liang, Zhangming Niu, Evandro Fei Fang, Yinhai Wang, Guang Yang

Abstract: Highlights•We introduce a novel diffusion model to synthesise fluorescence images from brightfield images.•CellResDM improves quality, speed, and segmentation accuracy, surpassing existing models.•CellResDM model can simultaneously generates IF images and cell/nuclei segmentation.