CRISPR/Cas9 cleavage efficiency regression through boosting algorithms and Markov sequence profiling

Hui Peng, Yi Zheng, Michael Blumenstein, Dacheng Tao, Jinyan Li

Abstract: CRISPR/Cas9 system is a widely used genome editing tool. A prediction problem of great interests for this system is: how to select optimal single-guide RNAs (sgRNAs), such that its cleavage efficiency is high meanwhile the off-target effect is low.