Abstract: Highlights•A pioneering work for cross-batch cell line identification with brightfield images.•Categorizing batch effects into three forms, offering tailored solutions for each.•Constructing a large-scale cell image dataset with 32 cell lines across 93 batches.•Accurate cell line identification even with single-batch training per class.
External IDs:doi:10.1016/j.media.2024.103123
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