Integrative Detection of Genome-Wide Translation Using iRibo

Alistair Turcan, Jiwon Lee, Aaron Wacholder, Anne-Ruxandra Carvunis

Abstract:

Ribosome profiling is a sequencing technique that provides a global picture of translation across
a genome. Here, we present iRibo, a software program for integrating any number of ribosome
profiling samples to obtain sensitive inference of annotated or unannotated translated open
reading frames. We describe the process of using iRibo to generate a species’ translatome from a
set of ribosome profiling samples using S. cerevisiae as an example.