Acquisition and Algorithms for Fluorescence Applications

Elena Mocanu, Paul E. Sterian

Abstract: The goal of this study is to present some important updates regarding the photo bleaching field. Fluorescence Recovery after Photobleaching (FRAP) is a versatile technique to determinediffusion coefficients of suitably labeled species in fields like pharmaceutical research, biophysics or macromolecular chemistry. Also we want to emphasize the importance of the numerical simulations especially when we want to extract features of the protein dynamics. The data are processed with Matlab and FEM programs. Of course the simulated situations have to be in a close agreement with the experimental data captured by an Andor Camera, iXON  + .