Analytical quality control in targeted lipidomics: Evaluating the performance of commercial plasma as a surrogate for pooled study samples

Alanah Grant-St James, Aude-Claire Lee, Alex J. Lee, Julien Wist, Ferdous Sohel, Kok Wai Wong, Bu B. Yeap, Ruey Leng Loo, Amanda Henry, Daniella Susic, Emad El-Omar, Jeremy K. Nicholson, Elaine Holmes, Luke Whiley, Nicola Gray

Abstract: Highlights•Comparison of two quality control workflows; pooled study and surrogate QC samples.•In-depth assessment of lipid composition, precision, and filtering.•OPLS-DA model predictive power maintained with both QC pre-processing strategies.•Surrogate QC samples are a robust alternative to a pooled QC in targeted lipidomics.

External IDs:doi:10.1016/j.aca.2025.344225