Rapid screening of multi-point mutations for enzyme thermostability modification by utilizing computational tools

Jia Jin, Qiaozhen Meng, Min Zeng, Guihua Duan, Ercheng Wang, Fei Guo

Abstract: Highlights•A comparison of multi-point mutated protein stability change predictors is conducted.•Datasets for single-point, double-point and multi-point mutations are collected.•Variants of IsPETase and LCC are used to test the effectiveness of predictors.•Available predictors for variants with specific amino acid mutation types are recommended.