Developing 3D microscopy with CLARITY on human brain tissue: Towards a tool for informing and validating MRI-based histology

Markus Morawski, Evgeniya Kirilina, Nico Scherf, Carsten Jäger, Katja Reimann, Robert Trampel, Filippos Gavriilidis, Stefan Geyer, Bernd Biedermann, Thomas Arendt, Nikolaus Weiskopf

Abstract: Highlights•CLARITY on post mortem human brain was adapted for validation of MRI-based histology.•Clearing of up to 8 mm, 3D imaging and staining of up to 5 mm thick samples was demonstrated.•Multiple stains were applied including markers for fibers, neurons and glia.•Characterization of cyto- and myelo-architectonics within an MRI-voxel was shown.