Split and merge watershed: A two-step method for cell segmentation in fluorescence microscopy images

Margarita Gamarra, Eduardo E. Zurek, Hugo Jair Escalante, Leidy Hurtado, Homero San-Juan-Vergara

Abstract: Highlights•A new cell segmentation algorithm using split and merge techniques was proposed.•Morphological features of the cells are used to separate clustered cells.•The algorithm preserves the shape of the cells and does not need training images.•SM-Watershed reached a balance between over-segmentation and under-segmentation.