pytom-match-pick: A tophat-transform constraint for automated classification in template matching

Marten L. Chaillet, Sander Roet, Remco C. Veltkamp, Friedrich Förster

Abstract: Highlights•pytom-match-pick is a GPU-accelerated software for template matching in cryo-electron tomograms.•A novel application of a tophat transform on score maps is tested and proven effective at filtering peaks.•The tophat transform is integrated into a dual-constraint thresholding strategy which improves classification of macromolecules in a simulated dataset.•In experimental data, the dual-constraint thresholding strategy leads to improved subtomogram averages.

External IDs:doi:10.1016/j.yjsbx.2025.100125