Blinking statistics and molecular counting in direct stochastic reconstruction microscopy (dSTORM)

Lekha Patel, David J. Williamson, Dylan M. Owen, Edward A. K. Cohen

Abstract: Many recent advancements in single-molecule localization microscopy exploit the stochastic photoswitching of fluorophores to reveal complex cellular structures beyond the classical diffraction limit. However, this same stochasticity makes counting the number of molecules to high precision extremely challenging, preventing key insight into the cellular structures and processes under observation.