Applications of Image Registration in Human Genome Research

Petr Matula, Michal Kozubek, Pavel Matula

Abstract: Fluorescence microscopy has some limitations and imperfections that can affect some types of study in human genome research. Image registration methods can help overcome or reduce the effect of some of them. This paper is concentrated on several applications of image registration that are carried out in our laboratory. The concerned areas are: 1. chromatic aberration correction, which improves precision of colocalization studies, 2. registration of images after repeated acquisitions, which helps to enlarge the number of object types studied simultaneously, and 3. registration of tilted images in micro-axial tomography, which helps to improve optical resolution of light microscopy.