3D Image Reconstruction from Multi-focus Microscopic Images

Takahiro Yamaguchi, Hajime Nagahara, Ken'ichi Morooka, Yuta Nakashima, Yuki Uranishi, Shoko Miyauchi, Ryo Kurazume

Keywords: 3D imaging, Microscopy, Multi-focus images, Transparent object

TL;DR: We propose a method for reconstructing 3D image from multi-focus microscopic images.

Abstract:

This paper presents a method for reconstructing 3D image
from multi-focus microscopic images captured with different focuses. We
model the multi-focus imaging by a microscopy and produce the 3D im-
age of a target object based on the model. The 3D image reconstruction
is done by minimizing the difference between the observed images and
the simulated images generated by the imaging model. Simulation and
experimental result shows that the proposed method can generate the
3D image of a transparent object efficiently and reliably.