DEGAST3D: Learning Deformable 3D Graph Similarity to Track Plant Cells in Unregistered Time Lapse Images

Md Shazid Islam, Arindam Dutta, Calvin-Khang Ta, Kevin Rodriguez, Christian Michael, Mark Alber, G. Venugopala Reddy, Amit K. Roy-Chowdhury

Abstract: Tracking plant cells in three-dimensional (3D) tissue captured through light microscopy presents significant challenges due to the large number of densely packed cells, non-uniform growth patterns, and variations in cell division planes across different cell layers. In addition, images of deeper tissue layers are often noisy, and systemic imaging errors further exacerbate the complexity of the task. In this paper, we propose a novel learning-based method DEGAST3D: Learning Deformable 3D GrAph Similarity to Track Plant Cells in Unregistered Time Lapse Images exploits the tightly packed 3D cell structure of plant cells to create a three-dimensional graph for accurate cell tracking. We also propose a novel algorithm for cell division detection and an effective three-dimensional registration, improving state-of-the-art algorithms. On a public dataset, our novel cell pair matching method outperforms the baseline by $6.83 \%$, $5.96 \%$, $6.40 \%$ in precision, recall, and F-1 score, respectively. On the same dataset, our proposed novel cell division technique improves the results of the baseline method by $15.38 \%$ and $14.78 \%$ in terms of recall and F1-score, respectively.

External IDs:doi:10.1109/tcbbio.2024.3525404