Automated fluorescence microscopy image analysis of Pseudomonas aeruginosa bacteria in alive and dead stadium

Marcin Wozniak, Dawid Polap, Leon Kosmider, Tomasz Clapa

Abstract: Highlights •Novel, efficient methodology for bacteria detection from fluorescent microscopy images.•Automated approach to analysis and calculation of bacteria, both in dead and alive stadium.•Experimental research results for Pseudomonas aeruginosa.•Epifluorescence microscope, filter set for Green Fluorescence Protein plasmid and for Propidium Iodide.