stDiffusion: A Diffusion Based Model for Generative Spatial Transcriptomics

Sumeer Ahmad Khan, Xabier Martínez de Morentin, Vincenzo Lagani, Robert Lehmann, Narsis A. Kiani, David Gomez-Cabrero, Jesper Tegnér

Track: Tiny paper track (up to 4 pages)

Abstract:

Spatial Transcriptomics (ST) allows deep characterization of the 2D organization of expression data within tissue slices. The ST technology provides a tissue contextualization of deep single-cell profiles. Recently, numerous computational and machine learning methods have addressed challenges such as data quality, augmentation, annotation, and the development of integrative platforms for data analysis. In contrast, here we ask whether unseen spatial transcriptomics data can be predicted and if we can interpolate novel transcriptomic slices. To this end, we adopt a denoising diffusion probabilistic-based model (DDPM) to demonstrate the learning of generative ST models for several tissues. Furthermore, our generative diffusion model interpolates (predicts) unseen slices located “between” the collected finite number of ST slices. This methodology sets the stage for learning predictive deep 3D models of tissues from a finite number of spatial transcriptomics slices, thus heralding the advent of AI-augmented spatial transcriptomics.

Submission Number: 81